First successful head-start program of leatherback sea turtles (Dermochelys coriacea) in Thailand and proposed dietary strategy.

Leatherback sea turtle (Dermochelys coriacea) hatchlings have previously been kept alive for over a year in captive conditions but these were the few survivors of initially larger groups. In this report, newly hatched leatherback sea turtles were randomly harvested from two nests in Thailand and successfully reared before release back to the wild. In captivity, the survival of the turtles decreased sharply during the first 4 or 5 months, and then steadied until the end of the 12-month trial. The survival of yearlings from one nest was 13.3% and from the other nest 46.7%. Their growth was exponential, reaching 1.36-3.35 kg (n = 9) at the end of program. The causes of death of the reared turtles were accidents (25.0-38.5%) and infections from water and live food (61.5-75.0%). The dietary requirements of the turtles were investigated by determining the digestibility of protein and carbohydrate in vitro, using enzymes from the digestive tracts of hatchlings that had died in an accident (70.1-92.9 g body weight). The results indicated that the most suitable source of protein was wheat gluten, followed by soybean meal, nonruminant meat and bone meal, fish meal, nonruminant meat meal, pork meal, and skimmed milk, while suitable carbohydrate sources were mashed cassava, corn, wheat flour, and alpha starch, followed by cereal meal and rice flour. Findings from the current report provide a practical protocol for head-starting leatherback sea turtles, and indicate the possible basis of an effective artificial diet for this species.

Dietary protein requirement for captive juvenile green turtles (Chelonia mydas).

Head-starting programs are extremely important for restoring the population of sea turtles in wild whereas husbandry conditions and feeding regimens of captive turtles are still limited. In the current study, the optimal dietary protein requirement for green turtle (Chelonia mydas) was investigated to support rearing in head-starting programs. Twenty-five-day-old turtles (44.5-46.2 g body weight, n = 45) were randomly distributed into 15 experimental plastic tanks, comprising three treatment replications of 3 turtles each. They were fed fishmeal-based feeds containing different levels of protein (30%, 35%, 40%, 45%, and 50%) for 8 weeks. At the end of feeding trial, growth performance (specific growth rate = 1.86% body weight/day) and feed utilization (protein efficiency ratio = 3.30 g gain/g protein) were highest in turtles fed with 40% protein in feed (p < .05). These nutritional responses were significantly supported by specific activities of fecal digestive enzymes, especially trypsin, chymotrypsin, amylase, and the amylase/trypsin ratio. Also, this dietary level improved the deposition of calcium and phosphorus in carapace, supporting a hard carapace and strong healthy bones. There were no negative effects in general health status of reared turtles, as indicated by hematological parameters. Based on a broken-line analysis between dietary protein levels and specific growth rate, the optimal protein level for green turtles was estimated as 40.6%. Findings from the current study support the use of artificial diets of specific protein levels to rear captive green turtle before release to natural habitats.

Enrichment devices for green turtles (Chelonia mydas) reared in captivity programs.

Environmental enrichment supports the well-being and welfare of captive animals. In the current study, the most suitable form of enrichment device for captive green turtles (Chelonia mydas) was investigated, to support head-start programs rearing turtles for release into their natural habitat. Fifteen-day-old turtles (113-114 g initial weight, n = 75) were randomly distributed into 15 experimental plastic tanks, comprising 5 treatments across three pools of each condition. The turtles in the experimental groups were exposed to four forms of enrichment devices (RS, ring shape; HSQS, hollow square shape; SS, sphere shape; CS, cylinder shape), and their outcomes related to growth, feed utilization, behavior, reduction of injury from conspecifics, and several health parameters were compared to those of a control group. At the end of the 10-week trial, the growth and feed utilization parameters did not differ across the five groups (p > .05). Of the turtles in the experimental treatments, those in the RS treatment spent more time interacting with the enrichment device, followed by the HSQS group. The percentage of wounds suffered through biting was significantly reduced in the groups exposed to enrichment devices, notably in the turtles exposed to the SS device, followed by the RS device. Significant differences between experimental groups in the specific activities of the major intestinal protein-digesting enzymes (trypsin and chymotrypsin) were observed. There were no effects noted in the hematological parameters and the main carapace elemental profile as compared to the control treatment. These findings suggest that the RS device is most appropriate in enriching the environment of juvenile green turtles in captivity programs, as well as in zoos or aquaria.

Optimal Feeding Frequency for Captive Hawksbill Sea Turtle (Eretmochelys imbricata).

Hawksbill sea turtle (Eretmochelys imbricata) has been reared in head-starting captivity programs, while the feeding regimens have never been optimized. In the current study, the feeding frequency of hawksbill sea turtle was investigated in indoor experimental conditions. Two-month-old turtles (38.98 ± 0.02 g) were distributed to triplicates of five treatments containing three turtles each and they were fed at different frequencies: one meal daily at 12.00 h (1M12), two meals daily at 08.00 and 12.00 h (2M8-12), two meals daily at 08.00 and 16.00 h (2M8-16), two meals daily at 12.00 and 16.00 h (2M12-16), or three meals daily at 08.00, 12.00 and 16.00 h (3M8-12-16). At the end of an 8-week trial, growth performance (specific growth rate 2.39 ± 0.02% body weight day-1) and feed consumption (feeding rate 2.00 ± 0.43 g day-1) were highest for turtles fed 2M8-16, followed by 2M12-16 or 3M8-12-16 relative to the other treatments (p ˂ 0.05). These treatments had significantly higher trypsin specific activity and trypsin/chymotrypsin ratio, and vice versa for lipase specific activity and amylase/trypsin ratio, relative to the remaining treatments. These match well with the fecal thermal properties that indicate amounts of available and unavailable nutrients present in the feces. Hematological parameters and carapace elemental composition showed no negative effects to turtles in 2M8-16 treatment. Therefore, two meals daily with long time interval were optimal for feeding hawksbill sea turtle. Findings from the current study could be directly used as a feeding guideline supporting the head-starting programs of this species.

Optimal Background Color for Head-Starting Northern River Terrapins (Batagur baska Gray, 1831).

Background color has significant effects on the lifestyles of various animal species. In this study, the effects of wall background color on growth, feed utilization, specific activity of gastric and pancreatic enzymes in fecal samples, fecal thermal properties, carapace elemental profile, and hematological parameters were investigated in northern river terrapin (Batagur baska Gray, 1831) in order to optimize the head-starting conditions. The terrapins (70.13 ± 0.04 g initial weight) were completely randomized into five types of colored tanks (transparent, green, red, blue, and black) and reared for twelve weeks. At the end of the experiment, tank color had no effect on survival and growth performance, but had significant effects on feeding rate, feed conversion ratio, and protein efficiency ratio (p < 0.05). Terrapins reared with black, red, or blue backgrounds had the highest feed utilization among the treatments. Among these three groups, analysis of specific activity of gastric and pancreatic enzymes in fecal samples and fecal thermal properties suggested improved digestive functionality in terrapins reared with a blue background relative to the other treatments. Carapace elemental composition and hematological parameters indicated no negative effects on health status of the terrapins reared with this optimal treatment. Findings from the current study support the head-starting program of northern river terrapins before release to natural habitats, and could also be applied in aquaria or zoos for public display.

5334000-53340000Optimal Salinity for Head-Starting Northern River Terrapins (Batagur baska Gray, 1831).

Northern river terrapins (Batagur baska Gray, 1831) are Asia's largest turtles living in both freshwater and brackish water. In the current study, the optimal salinity for head-starting programs of this critically endangered species was investigated in order to serve the well-being of turtles before release to natural habitat. Forty-eight terrapins (54.64 ± 0.18 g initial body weight) were randomly distributed to four salinity levels (0, 4, 8, and 12 ppt) and reared for eight weeks, using three replicates with four terrapins each. At the end of rearing trial, growth performance and feed utilization parameters were superior in terrapins reared at 4 ppt, followed by 8 ppt in the rank order of treatments. Negative stress responses were observed in terrapins reared at 12 ppt, as the fecal activity of amylase-to-trypsin ratio was changed significantly, but not that of proteolytic enzymes. The fecal thermal transition properties indicated an abundance of nutrients in the post-absorptive phase for terrapins reared at 4 ppt, followed by the 8 ppt treatment group. The preferred 4 ppt salinity had no negative effects on the health status of the terrapins in terms of carapace elemental composition or hematological parameters. Second-order polynomial regression suggests 4.35 ppt as the optimal salinity for maximal weight gain. Findings from the current study could be directly used in ex situ conservation programs of northern river terrapins before release to natural habitat.

Optimal-rearing density for head-starting green turtles (Chelonia mydas Linnaeus, 1758).

While ex situ conservation programs of juvenile green turtles (Chelonia mydas Linnaeus, 1758), before release to natural habitats, have been conducted in several countries, the optimal-stocking density for husbandry has not yet been reported. The optimization of stocking density was the main purpose of this study. The 15-day-old post-hatching turtles (29.30 ± 0.05 g body weight) were reared in round fiberglass tanks at various stocking densities including 20 turtles/m3 (20TM), 40 turtles/m3 (40TM), 60 turtles/m3 (60TM), and 80 turtles/m3 (80TM), over an 8-week trial. Water quality, survival, growth performance, feed utilization, aggressive behavior, fecal digestive enzymes, and hematological parameters were compared between the treatments, and were used as indicators of a successful captive rearing program. The water quality across the four treatments was in the standard range, but a high-stocking density reduced the quality significantly. No mortality was observed in any treatment group. Superior growth and feed utilization were only observed with the 40TM treatment, relative to the others (P < 0.05). The turtles in this group had no aggressive behavior, as indicated by observing hind limb biting. This treatment manipulated the level of proteolytic activity of pepsin and trypsin in response to density stressor, but not amylase, lipase, and chymotrypsin. The 40TM treatment also maintained the hematological characteristics, indicating no negative effects on health status. Overall, the findings indicate that the captivity program of post-hatching turtles at 40 turtles/m3 is the preferred option in their head-started propagation, as well as in public displays in zoos or aquaria. Zoo Biol. 35:454-461, 2016. © Wiley Periodicals, Inc.